Table 2.
Mean, upper and lower limits of 95% reference intervals are reported for phyla, families, and genera with abundance > 0.1%.
| Total | Cluster 1 | Cluster 2 | |
|---|---|---|---|
| Phyla | |||
| P: Firmicutes | 46.45% (18.05–66.11) | 26.99% (3.75–37.38) | 50.21% (36.53–66.01) |
| P: Bacteroidetes | 43.17% (20.94–89.29) | 57.55% (21.76–80.83) | 40.38% (17.8–56.85) |
| P: Proteobacteria | 6.15% (0.56–30.17) | ||
| P: Verrucomicrobiota | 1.6% (0–17.41) | ||
| P: Cyanobacteria | 1.16% (0–21.28) | ||
| P: Actinobacteriota | 0.6% (0–4.74) | 0.3% (0–3.16) | 0.66% (0–4.83) |
| P: Fusobacteriota | 0.29% (0–2.45) | ||
| P: Euryarchaeota | 0.24% (0–4.27) | ||
| P: Desulfobacterota | 0.17% (0–1.59) | ||
| Families | |||
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Bacteroidaceae | 25.93% (1.09–67.09) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae | 21.8% (3.22–41.47) | 13.55% (3.32–23.15) | 23.39% (4.42–41.24) |
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Prevotellaceae | 10.45% (0–73.71) | ||
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Ruminococcaceae | 9.96% (0.56–27.93) | 6.08% (0–13.42) | 10.71% (0.32–28.56) |
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Oscillospiraceae | 2.53% (0.01–15.98) | 0.98% (0–5.43) | 2.83% (0.12–15.49) |
| P: Proteobacteria; C: Gammaproteobacteria; O: Burkholderiales; F: Sutterellaceae | 2.26% (0–10.88) | ||
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Tannerellaceae | 2.21% (0–12.91) | ||
| P: Proteobacteria; C: Alphaproteobacteria; O: Rhodospirillales; F: uncultured | 2.18% (0–17.76) | ||
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Rikenellaceae | 2.16% (0–9.91) | ||
| P: Firmicutes; C: Clostridia; O: Clostridia_UCG-014; F: Clostridia_UCG-014 | 1.94% (0–13.12) | 0.64% (0–4.63) | 2.19% (0–13.12) |
| P: Verrucomicrobiota; C: Verrucomicrobiae; O: Verrucomicrobiales; F: Akkermansiaceae | 1.51% (0–17.41) | ||
| P: Firmicutes; C: Negativicutes; O: Acidaminococcales; F: Acidaminococcaceae | 1.28% (0–8.02) | ||
| P: Proteobacteria; C: Gammaproteobacteria; O: Enterobacterales; F: Enterobacteriaceae | 1.24% (0–31.06) | ||
| P: Cyanobacteria; C: Vampirivibrionia; O: Gastranaerophilales; F: Gastranaerophilales | 1.15% (0–21.28) | ||
| P: Firmicutes; C: Clostridia; O: Clostridia_vadinBB60_group; F: Clostridia_vadinBB60_group | 1.15% (0–8.73) | 0.24% (0–1.85) | 1.32% (0–9.76) |
| P: Firmicutes; C: Clostridia; O: Christensenellales; F: Christensenellaceae | 1.1% (0–7.76) | 0.3% (0–6.38) | 1.25% (0–8.44) |
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Barnesiellaceae | 1.03% (0–5.14) | ||
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: [Eubacterium]_coprostanoligenes_group | 0.88% (0–5.58) | 0.39% (0–2.72) | 0.97% (0–6.59) |
| P: Firmicutes; C: Negativicutes; O: Veillonellales-Selenomonadales; F: Veillonellaceae | 0.87% (0–6.53) | ||
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Marinifilaceae | 0.75% (0–2.38) | ||
| P: Firmicutes; C: Bacilli; O: Erysipelotrichales; F: Erysipelatoclostridiaceae | 0.74% (0.02–5.48) | 0.28% (0–1.88) | 0.83% (0.01–8.81) |
| P: Firmicutes; C: Clostridia; O: Monoglobales; F: Monoglobaceae | 0.7% (0–3.66) | ||
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: UCG-010 | 0.68% (0–5.74) | 0.3% (0–9.27) | 0.76% (0–6.48) |
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Muribaculaceae | 0.56% (0–6.46) | ||
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Butyricicoccaceae | 0.48% (0.02–2.08) | 0.24% (0–1.07) | 0.53% (0.01–2.04) |
| P: Actinobacteriota; C: Actinobacteria; O: Bifidobacteriales; F: Bifidobacteriaceae | 0.48% (0–3.67) | 0.22% (0–2.71) | 0.53% (0–4.24) |
| P: Firmicutes; C: Bacilli; O: Erysipelotrichales; F: Erysipelotrichaceae | 0.36% (0–3.93) | ||
| P: Firmicutes; C: Bacilli; O: Izemoplasmatales; F: Izemoplasmatales | 0.35% (0–4.88) | 0.02% (0–0.44) | 0.41% (0–6.95) |
| P: Firmicutes; C: Clostridia; O: Peptostreptococcales-Tissierellales; F: Peptostreptococcaceae | 0.3% (0–1.96) | ||
| P: Fusobacteriota; C: Fusobacteriia; O: Fusobacteriales; F: Fusobacteriaceae | 0.29% (0–2.45) | ||
| P: Proteobacteria; C: Gammaproteobacteria; O: Pasteurellales; F: Pasteurellaceae | 0.26% (0–2.86) | ||
| P: Euryarchaeota; C: Methanobacteria; O: Methanobacteriales; F: Methanobacteriaceae | 0.24% (0–4.19) | ||
| P: Firmicutes; C: Clostridia; O: Clostridiales; F: Clostridiaceae | 0.23% (0–1.49) | ||
| P: Firmicutes; C: Bacilli; O: Acholeplasmatales; F: Acholeplasmataceae | 0.23% (0–4.75) | ||
| P: Firmicutes; C: Bacilli; O: Lactobacillales; F: Streptococcaceae | 0.19% (0–1.65) | ||
| P: Firmicutes; C: Bacilli; O: RF39; F: RF39 | 0.18% (0–2.99) | 0.02% (0–0.41) | 0.21% (0–3.06) |
| P: Desulfobacterota; C: Desulfovibrionia; O: Desulfovibrionales; F: Desulfovibrionaceae | 0.17% (0–1.57) | ||
| P: Proteobacteria; C: Gammaproteobacteria; O: Aeromonadales; F: Succinivibrionaceae | 0.15% (0–0.05) | ||
| P: Firmicutes; C: Clostridia; O: Peptostreptococcales-Tissierellales; F: Anaerovoracaceae | 0.14% (0–0.93) | ||
| Genera | |||
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Bacteroidaceae; G: Bacteroides | 25.93% (1.09–67.09) | ||
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Prevotellaceae; G: Prevotella | 7.98% (0–70.33) | ||
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Ruminococcaceae; G: Faecalibacterium | 4.07% (0.34–20.59) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Unclassified | 2.61% (0.08–7.66) | 1.51% (0–4.36) | 2.82% (0.31–7.83) |
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Roseburia | 2.58% (0–12.04) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Lachnospira | 2.57% (0–15.38) | ||
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Tannerellaceae; G: Parabacteroides | 2.21% (0–12.78) | ||
| P: Proteobacteria; C: Alphaproteobacteria; O: Rhodospirillales; F: uncultured; G: uncultured | 2.18% (0–17.76) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Blautia | 2.02% (0.31–7.38) | ||
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Rikenellaceae; G: Alistipes | 1.97% (0–9.89) | ||
| P: Firmicutes; C: Clostridia; O: Clostridia_UCG-014; F: Clostridia_UCG-014; G: Clostridia_UCG-014 | 1.94% (0–13.28) | 0.64% (0–4.63) | 2.19% (0–13.12) |
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Ruminococcaceae; G: Ruminococcus | 1.9% (0–12.56) | 1.1% (0–12.59) | 2.05% (0–16.77) |
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Lachnospiraceae_NK4A136_group | 1.83% (0–11.57) | 0.97% (0–7.16) | 2% (0–11.99) |
| P: Verrucomicrobiota; C: Verrucomicrobiae; O: Verrucomicrobiales; F: Akkermansiaceae; G: Akkermansia | 1.51% (0–17.41) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: [Eubacterium]_eligens_group | 1.42% (0–8.07) | 0.75% (0–4.08) | 1.55% (0–8.3) |
| P: Proteobacteria; C: Gammaproteobacteria; O: Burkholderiales; F: Sutterellaceae; G: Sutterella | 1.36% (0–6.15) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Agathobacter | 1.16% (0–6.4) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Coprococcus | 1.15% (0–7.53) | 0.47% (0–2.96) | 1.28% (0–7.98) |
| P: Cyanobacteria; C: Vampirivibrionia; O: Gastranaerophilales; F: Gastranaerophilales; G: Gastranaerophilales | 1.15% (0–21.28) | ||
| P: Firmicutes; C: Negativicutes; O: Acidaminococcales; F: Acidaminococcaceae; G: Phascolarctobacterium | 1.15% (0–8.55) | ||
| P: Firmicutes; C: Clostridia; O: Clostridia_vadinBB60_group; F: Clostridia_vadinBB60_group; G: Clostridia_vadinBB60_group | 1.15% (0–8.73) | 0.24% (0–1.85) | 1.32% (0–9.76) |
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Ruminococcaceae; G: [Eubacterium]_siraeum_group | 1.13% (0–9.32) | ||
| P: Proteobacteria; C: Gammaproteobacteria; O: Enterobacterales; F: Enterobacteriaceae; G: Escherichia-Shigella | 1.13% (0–28.31) | ||
| P: Firmicutes; C: Clostridia; O: Christensenellales; F: Christensenellaceae; G: Christensenellaceae_R-7_group | 1.08% (0–7.63) | 0.29% (0–6.35) | 1.23% (0–8.17) |
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Prevotellaceae; G: Prevotellaceae_NK3B31_group | 1.07% (0–18.99) | ||
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Oscillospiraceae; G: UCG-002 | 1.04% (0–9.18) | 0.3% (0–2.77) | 1.18% (0–11.44) |
| P: Proteobacteria; C: Gammaproteobacteria; O: Burkholderiales; F: Sutterellaceae; G: Parasutterella | 0.91% (0–12.06) | ||
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: [Eubacterium]_coprostanoligenes_group; G: [Eubacterium]_coprostanoligenes_group | 0.88% (0–5.58) | 0.39% (0–2.72) | 0.97% (0–6.59) |
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Ruminococcaceae; G: Subdoligranulum | 0.84% (0–4.84) | 0.33% (0–3.53) | 0.94% (0–4.88) |
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Barnesiellaceae; G: Barnesiella | 0.79% (0–3.99) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Fusicatenibacter | 0.73% (0–4.78) | 0.31% (0–3.19) | 0.82% (0.01–5.18) |
| P: Firmicutes; C: Negativicutes; O: Veillonellales-Selenomonadales; F: Veillonellaceae; G: Dialister | 0.7% (0–6.14) | ||
| P: Firmicutes; C: Clostridia; O: Monoglobales; F: Monoglobaceae; G: Monoglobus | 0.7% (0–3.65) | ||
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: UCG-010; G: UCG-010 | 0.68% (0–5.74) | 0.3% (0–9.27) | 0.76% (0–6.5) |
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Ruminococcaceae; G: Unclassified | 0.68% (0–5.09) | ||
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Ruminococcaceae; G: CAG-352 | 0.65% (0–7.56) | ||
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Prevotellaceae; G: Alloprevotella | 0.62% (0–16.06) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: [Eubacterium]_ruminantium_group | 0.59% (0–8.19) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Anaerostipes | 0.55% (0–3.16) | 0.26% (0–0.69) | 0.61% (0.01–3.43) |
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Muribaculaceae; G: Muribaculaceae | 0.55% (0–6.47) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Dorea | 0.54% (0–1.88) | ||
| P: Actinobacteriota; C: Actinobacteria; O: Bifidobacteriales; F: Bifidobacteriaceae; G: Bifidobacterium | 0.48% (0–3.64) | 0.22% (0–2.71) | 0.53% (0–4.24) |
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Lachnoclostridium | 0.46% (0–2.32) | ||
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Marinifilaceae; G: Odoribacter | 0.46% (0–1.76) | ||
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Prevotellaceae; G: Paraprevotella | 0.46% (0–3.83) | ||
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Oscillospiraceae; G: UCG-005 | 0.45% (0–2.71) | 0.17% (0–3.99) | 0.5% (0–2.64) |
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Butyricicoccaceae; G: Butyricicoccus | 0.43% (0–2.16) | 0.24% (0–1.07) | 0.47% (0–2.19) |
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Ruminococcaceae; G: uncultured | 0.39% (0–2.45) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Lachnospiraceae_UCG-001 | 0.39% (0–3.69) | 0.14% (0–1.19) | 0.44% (0–3.78) |
| P: Firmicutes; C: Bacilli; O: Erysipelotrichales; F: Erysipelatoclostridiaceae; G: Erysipelotrichaceae_UCG-003 | 0.38% (0–2.04) | 0.18% (0–1.96) | 0.42% (0–2) |
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Lachnospiraceae_UCG-003 | 0.37% (0–10.84) | ||
| P: Firmicutes; C: Bacilli; O: Izemoplasmatales; F: Izemoplasmatales; G: Izemoplasmatales | 0.35% (0–4.86) | 0.02% (0–0.44) | 0.41% (0–6.95) |
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: [Eubacterium]_xylanophilum_group | 0.35% (0–1.58) | 0.1% (0–1.35) | 0.4% (0–1.62) |
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Oscillospiraceae; G: UCG-003 | 0.33% (0–1.4) | 0.14% (0–1.49) | 0.36% (0–1.52) |
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Butyrivibrio | 0.32% (0–15.4) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: [Eubacterium]_hallii_group | 0.3% (0–0.97) | 0.16% (0–0.86) | 0.32% (0.01–1.1) |
| P: Fusobacteriota; C: Fusobacteriia; O: Fusobacteriales; F: Fusobacteriaceae; G: Fusobacterium | 0.29% (0–2.45) | ||
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Marinifilaceae; G: Butyricimonas | 0.28% (0–1.17) | ||
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Oscillospiraceae; G: NK4A214_group | 0.27% (0–3) | 0.04% (0–0.32) | 0.31% (0–4.19) |
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Tyzzerella | 0.27% (0–4.34) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: [Ruminococcus]_torques_group | 0.26% (0.01–2) | ||
| P: Proteobacteria; C: Gammaproteobacteria; O: Pasteurellales; F: Pasteurellaceae; G: Haemophilus | 0.25% (0–2.78) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: [Eubacterium]_ventriosum_group | 0.24% (0–1.32) | 0.1% (0–1.35) | 0.27% (0–1.34) |
| P: Firmicutes; C: Bacilli; O: Acholeplasmatales; F: Acholeplasmataceae; G: Anaeroplasma | 0.23% (0–4.75) | ||
| P: Firmicutes; C: Clostridia; O: Clostridiales; F: Clostridiaceae; G: Clostridium_sensu_stricto_1 | 0.23% (0–1.5) | ||
| P: Firmicutes; C: Bacilli; O: Erysipelotrichales; F: Erysipelotrichaceae; G: Holdemanella | 0.23% (0–2.68) | ||
| P: Euryarchaeota; C: Methanobacteria; O: Methanobacteriales; F: Methanobacteriaceae; G: Methanobrevibacter | 0.22% (0–4.14) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Lachnospiraceae_UCG-004 | 0.19% (0–1.05) | ||
| P: Firmicutes; C: Bacilli; O: Erysipelotrichales; F: Erysipelatoclostridiaceae; G: Asteroleplasma | 0.19% (0–5.16) | ||
| P: Firmicutes; C: Bacilli; O: Lactobacillales; F: Streptococcaceae; G: Streptococcus | 0.18% (0–1.66) | ||
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Rikenellaceae; G: Rikenellaceae_RC9_gut_group | 0.18% (0–2.95) | ||
| P: Firmicutes; C: Bacilli; O: RF39; F: RF39; G: RF39 | 0.18% (0–2.92) | 0.02% (0–0.41) | 0.21% (0–3.06) |
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Lachnospiraceae_ND3007_group | 0.17% (0–0.79) | 0.06% (0–0.37) | 0.19% (0–0.94) |
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: Lachnospiraceae_UCG-010 | 0.17% (0–0.87) | 0.08% (0–0.98) | 0.18% (0–1.02) |
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Ruminococcaceae; G: Incertae_Sedis | 0.15% (0–1.14) | 0.08% (0–0.51) | 0.17% (0–1.26) |
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Prevotellaceae; G: uncultured | 0.15% (0–3.18) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: CAG-56 | 0.15% (0–1.07) | ||
| P: Firmicutes; C: Clostridia; O: Peptostreptococcales-Tissierellales; F: Peptostreptococcaceae; G: Romboutsia | 0.15% (0–1.1) | 0.07% (0–0.49) | 0.16% (0–1.42) |
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Prevotellaceae; G: Prevotellaceae_UCG-001 | 0.14% (0–3.37) | ||
| P: Proteobacteria; C: Gammaproteobacteria; O: Aeromonadales; F: Succinivibrionaceae; G: Succinivibrio | 0.14% (0–0) | ||
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Barnesiellaceae; G: uncultured | 0.12% (0–1.67) | ||
| P: Firmicutes; C: Negativicutes; O: Acidaminococcales; F: Acidaminococcaceae; G: Acidaminococcus | 0.12% (0–2.86) | ||
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Oscillospiraceae; G: Colidextribacter | 0.12% (0–0.59) | ||
| P: Bacteroidetes; C: Bacteroidia; O: Bacteroidales; F: Barnesiellaceae; G: Coprobacter | 0.11% (0–0.92) | ||
| P: Firmicutes; C: Clostridia; O: Oscillospirales; F: Oscillospiraceae; G: Oscillibacter | 0.11% (0–0.58) | ||
| P: Firmicutes; C: Clostridia; O: Peptostreptococcales-Tissierellales; F: Peptostreptococcaceae; G: Intestinibacter | 0.11% (0–1.03) | ||
| P: Firmicutes; C: Clostridia; O: Lachnospirales; F: Lachnospiraceae; G: [Ruminococcus]_gauvreauii_group | 0.1% (0–0.66) | ||
The reference intervals are reported for C1 and C2 if the abundance between the two clusters was significantly different (Mann–Whitney test with false discovery rate).
P phylum, C class, O order, F family, G genus.