Table 2.
Amino acid sequence alignment of human CCL2, CCL7, CCL8, CCL13, CCL16, PSMP, mouse CCL12, and their homologies with CCL2, binding affinities to CCR2.
| Chemokine | Amino acid sequences | Identities with CCL2 (%) | Affinities to CCR2 (Kd) | Refs |
|---|---|---|---|---|
| CCL2 | MKVSAALLCLLLIAATFIPQGLA QPDAINAPVT | 100 | ~0.5 nM | (34, 53) |
| CCYNFTNRKISVQRLASYRRITSSKCPKEAVIFK | ||||
| TIVAKEICADPKQKWVQDSMDHLDKQTQTPKT | ||||
| CCL7 | MKASAALLCLLLTAAAFSPQGLA QPVGINTSTT | 73 | ~13 nM | (35, 74) |
| CCYRFINKKIPKQRLESYRRTTSSHCPREAVIFK | ||||
| TKLDKEICADPTQKWVQDFMKHLDKKTQTPKL | ||||
| CCL8 | MKVSAALLCLLLMAATFSPQGLA QPDSVSIPIT | 69 | 3±1 nM | (35, 75) |
| CCFNVINRKIPIQRLESYTRITNIQCPKEAVIFKT | ||||
| KRGKEVCADPKERWVRDSMKHLDQIFQNLKP | ||||
| mCCL12 | MKISTLLCLLLIATTISPQVLA GPDAVSTPVT | 68 | ~5 nM | (36, 76) |
| CCYNVVKQKIHVRKLKSYRRITSSQCPREA | ||||
| VIFRTILDKEICADPKEKWVKNSINHLDKTS | ||||
| QTFILEPSCLG | ||||
| CCL13 | MKVSAVLLCLLLMTAA FNPQGLAQPDALNVPST | 65 | ~15 nM | (37, 77) |
| CCFTFSSKKISLQRLKSYVITTSRCPQKAVIFRTKLGKEICADPKEKWVQNYMKHLGRKAHTLKT | ||||
| CCL16 | MKVSEAALSLLVLILIITSASRS QPKVPEWVNTPST | 31 | ~95 nM | (38, 78) |
| CCLKYYEKVLPRRLVVGYRKALNCHLPAIIFVTKRNREVCTNPNDDWVQEYIKDPNLPLLPTRNLSTVKIITAKNGQPQLLNSQ | ||||
| PSMP | MALRMLWAGQAKGILGGWGIICLVMSLLLQHPGVYS KCYFQAQAPCHYEGKYFTLGESWLRKDCFHCTCLHPVGVGCCDTSQHPIDFPAGCEVRQEAGTCQFSLVQKSDPRLPCKGGGPDPEWGSANTPVPGAPAPHSS | – | ~1.4 nM | (40) |
The sequence data of each ligand of CCR2 is avaibable from GenBank, respectively. Conserved cysteine residues are indicated by the red font; N-terminal signal peptide sequence of the mature proteins are indicated by the blue font.