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. Author manuscript; available in PMC: 2023 Mar 17.
Published in final edited form as: Cell Chem Biol. 2021 Sep 15;29(3):476–489.e6. doi: 10.1016/j.chembiol.2021.08.014

Figure 1. Crude metabolite extracts stimulate ScTop2 activity.

Figure 1.

(A) Schematic of metabolite extraction procedure. (B-G) Representative gels and graphs of mean ± SD (n=3) for decatenation assays (B-D) and supercoil relaxation assays (E-G). Bands representing nicked minicircles and closed minicircles are indicated (B-C), as are bands representing unrelaxed substrate (SC), the relaxed topoisomer distribution, and nicked/open circle (OC) plasmids (E-F). No enzyme (‘-topo’) and no ATP (‘-ATP’) negative controls show the starting substrate. Metabolite extract and spent media were titrated from 0 to 50 mg/ml in 10 mg/ml increments.