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. Author manuscript; available in PMC: 2023 Mar 17.
Published in final edited form as: Cell Chem Biol. 2021 Sep 15;29(3):476–489.e6. doi: 10.1016/j.chembiol.2021.08.014

Figure 4. TCA cycle intermediates stimulate strand passage by topo II without significant effects on ATP hydrolysis.

Figure 4.

(A-H) Stimulation of topo II decatenation activity (A-D) and supercoil relaxation activity (E-H) by citrate and oxaloacetate (OAA). Metabolites were titrated from 0 to 40mM in 5mM increments. Graphs (B, D, F, and H) represent mean ± SD of n=3–5. (I-J) Stimulation of ScTop2 decatenation (I) and relaxation (J) activity by TCA metabolites and glutamate (negative control). Stimulation values represent mean of n=3–6 independent experiments. (See also Figure S3). (K-L) Effects of TCA metabolites on ATP hydrolysis activity. Km (K) and Vmax (μmol ATP • min−1 • nmol topo II−1) (L) values were derived from 3 replicates. (See also Figure S6).