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Citrate and succinate were titrated from 0 to 40 mM in 5 mM increments and added to decatenation assays with HsTop2B (A-D), HsTop2A (E-H), and Ec topo IV (I-L), and to supercoil relaxation assays with Sc topo I. Graphs represent mean ± SD of n=3–5.
