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. 2021 Mar 23;15(3):967–984. doi: 10.1111/1751-7915.13803

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© 2021 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Fig. 1 — Fitness of the individual and propagated lines under ethanol.

A. Experimental evolution strategy in 10 replicated lines under YNB + glucose (GLU, red tubes) and YNB + GLU + ethanol 9% (EtOH, blue tubes). From every line, individuals were isolated and subjected to phenotyping, fermentation and sequencing analysis.

B. The growth rate (µmax) of the different parental strains used in this study was estimated under ethanol 8%, 9% and 10%. The fitness of the propagated lines under (C) ethanol and (D) glucose.