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. 2021 Mar 23;15(3):967–984. doi: 10.1111/1751-7915.13803

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© 2021 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Fig. 5 — Differential gene expression analysis between the EtOH‐evolved CLEt5.1 strain and its native parental strain under beer wort fermentation conditions. The transcriptome of the EtOH‐evolved CLEt5.1 strain was evaluated and compared against the CL248.1 native strain under beer wort fermentation conditions.

A. The volcano plot depicts differentially expressed genes between CLEt5.1 and CL248.1.

B. Relative growth rates of CLEt5.1 and CL248.1 strains shifted from two 24 hours 5% glucose pre‐cultures to 5% maltose and 5% galactose media.

C. Network analysis in upregulated genes in CLEt5.1 depicting the most relevant hubs differently regulating genes between CLEt5.1 and CL248.1. Transcription factors are shown in red triangles, while TF‐gene connections are shown in red lines.