Fig. 3.
Differences of fat metabolism levels in liver between high and low body weight chickens. Chickens (Turpan cockfighting × White Leghorn) at 7th week of age were sacrificed to analyse fat metabolism level in liver (n = 10). Each group contained five males and five female chickens.
A. Fat content of hepatocytes (H & E staining). The white dots indicated by the arrows in the figure are fat droplets.
B. The relative mRNA expression of fat anabolism‐related genes (qPCR).
C. The relative mRNA expression of fat catabolism‐related genes (qPCR).
D. The relative mRNA expression of fat transport‐related genes (qPCR).
E. The distribution of P‐AMPK (IHC). The arrows in the figure indicated positive expression of P‐AMPK.
F. IOD comparison of IHC.
G–I. The protein expression of P‐AMPK (western blot). Scale bars = 50 μm. All data were presented as the means ± SEM. P values were calculated using Student’s t‐test, *P < 0.05, **P < 0.01.