Fig. 1.

Lipoteichoic acid (LTA) from BPL1 strain demonstrates fat‐reducing activity. A. Fat‐reducing effect of purified LTA at different doses. B. Representative images of Nile red staining of lipid content in live young adult C. elegans in a wild‐type N2 animal under fluorescence microscopy. Nematodes were fed with BPL1 cells, heat‐treated BPL1 cells (HT‐BPL1) or LTA (10 µg ml^‐1). Scale bar 250 µm. Original image taken by the authors for this paper with a Nikon‐SMZ18 Fluorescence Stereomicroscope. C. Quantification of triglyceride content (mM TG/mg protein) in C. elegans fed with purified LTA from BPL1. D. Analysis of fat‐reducing activity of LTAs from B. longum ES1 and B. animalis subsp. lactis BB12. For a, b percentage of fluorescence in bacterial and LTA‐fed nematodes (wild‐type strain N2) is represented. Nile red was quantified at young adult stage. Orlistat (6 µg ml^‐1) was used as positive control. Data are mean ± SD. and were calculated from two biological independent experiments. For A, B, C, D *P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant.