Fig. 3.

Lipoteichoic acid (LTA) from BPL1 strain requires the Insulin‐like signalling pathway (IGF‐I) to exert its fat‐reducing effect, and has functional activity in hyperglycaemic conditions. A. Feeding worms with LTA in mutant daf‐2 and daf‐16 strains; the same as with the live BPL1 cells and the heat‐treated BPL1 cells. B. Fat content in C. elegans mutant for SKN‐1 transcription factor treated with LTA from BPL1. C. Effect of treatments on a C. elegans hyperglycaemic model. Nematodes of wild‐type strain N2 grown in high glucose (100 mM). Metformin was used as positive control. For A, B, C percentage of fluorescence in LTA‐fed nematodes (wild‐type strain N2, GR1307, daf‐16 (mgDf50), CB1370, daf‐2 (e1370), or LG333 Skn‐1 (zu 135)). Nile red staining was quantified at young adult stage. Orlistat (6 µg ml⁻¹) was used as positive control. Data are mean ± SD. and were calculated from two independent biological experiments. ***P < 0.001, NS: not significant.