Fig. 4. Effects of scutellarin on the proinflammatory factor mRNA levels and iNOS activity in the cerebellum caused by alcohol exposure. (a‍‒‍c) The cDNA template was obtained by reverse transcription using mRNA extracted from the tissues, and the mRNA expression levels of IL-1β (a), IL-6 (b), and TNF ‍ - ‍ α (c) in cerebellar tissues were detected by qPCR. (d) The activity of iNOS in the supernatants of cerebellum tissue homogenate was determined by a biochemical kit. Data were presented as mean±SD (n=6). ^** P<0.01, ^*** P<0.001, vs. the control group. ^# P<0.05, ^## P<0.01, ^### P<0.001, vs. the alcohol group. n.s.: no significance (P>0.05). mRNA: messenger RNA; cDNA: complementary DNA; IL-1β: interleukin-1β; IL-6: interleukin-6; TNF ‍ - ‍ α: tumor necrosis factor-‍α; qPCR: quantitative polymerase chain reaction; iNOS: inducible nitric oxide synthase; SD: standard deviation.