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. 2022 Feb 25;12:739976. doi: 10.3389/fonc.2022.739976

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Copyright © 2022 Jiang, Li, Jiang, Li, Liu, Xu, Li, Feng and Zheng

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tim-3 expression in primary NK cells and NK cell lines. Tim-3 expression was quantified by flow cytometry. (A) Tim-3 expression in the BM and peripheral blood NK cells of MM patients and control donors. Freshly isolated hPBMCs and hBMMCs were stained with CD3, CD56, Tim-3, and analyzed for the proportion of Tim-3⁺NK cells. (B, C) Statistical analysis of the proportion of Tim-3⁺NK cells and the MFI of Tim-3 in NK cells (n=8). (D–F) The expression of Tim-3 in exNK, NKL and NK-92 cells. One representative results from at least three independent experiments were presented. Data shown were mean ± SD of at least three independent experiments. *P < 0.05, **P < 0.01 versus control NK cells.