Figure 2.

The expression of Tim-3 ligands in primary MM cells and MM cell lines. Tim-3 ligands expression were quantified by flow cytometry. (A) CD138⁺ MM cells were sorted by immunomagnetic separation from the bone marrow of MM patients and stained with Galetin-9, HMGB1, CEACAM1 for the proportion of Tim-3 ligands⁺MM cells. (B) Statistical analysis of the proportion and MFI of Tim-3 ligands⁺MM cells (n=5). (C, D) The expression of Tim-3 ligands in MM cell line RPMI8226 cells. (E, F) The expression of Tim-3 ligands in MM cell line MM.1S cells. Representative results from one of at least three independent experiments were exhibited. Data shown were mean ± SD of at least three independent experiments.