Figure 4.

Tim-3 blockade enhanced perforin and Granzyme B expression as well as cytokine secretion in NK cells. (A, B) exNK, NKL, NK-92 cells were pre-incubated with 10 μg/ml anti-Tim-3 or isotype antibodies for 48h. The protein levels of perforin and Granzyme B in NK cells were analyzed by flow cytometry. One representative of at least three independent experiments. Data shown were mean ± SD of at least three independent experiments. *P < 0.05, **P < 0.01 versus control NK cells. (C) exNK, NKL, NK-92 cells were pre-incubated with 10 μg/ml anti-Tim-3 or isotype antibodies. Production of TNF-α and IFN-γ by NK cells after stimulation with/no PMA/ionomycin for 48 h, as evaluated by ELISA assay. Data shown were mean ± SD of at least three independent experiments. *P < 0.05, **P < 0.01 versus control NK cells.