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Tim-3 ligands combined blockade enhanced NK cell-mediated natural cytotoxicity against human MM cell lines. exNK was used as effector (E) cells and human MM cell lines were used as target (T) cells. MM cell lines were pre-incubated by 10 μg/ml anti-Tim-3 ligands antibody. Cytotoxicity activity of NK cells was tested by PKH26/Annexin-V stain. *P < 0.05 versus control NK cells. (A) Cytotoxicity activity of NK cells. (B) Statistical Analysis of (A).
