Figure 7.

Anti-tumor effects of Tim-3 blockade NK cells on human MM tumor-bearing NOD/SCID mice. (A–C) Female NOD/SCID mice were subcutaneously injected with 1×10⁷ RPMI8226 cells and then intravenously injected with 1×10⁸ exNK cells with anti-Tim3 or isotype antibodies on day 7 and 14 post of tumor cell inoculation. The tumor volumes of tumor-bearing mice were measured every 3 days. The tumor burden was monitored by bioluminescence imaging with the IVIS system and the total tumor luminescence (photons/sec) of the tumors were measured. (D) NOD/SCID mice were i.v. injected with 2×10⁶ RPMI8226 cells and then injected intravenously with 2×10⁷ exNK cells with anti-Tim3 or isotype antibodies on day 7 and 14 post of tumor cell inoculation. The curves for the survival were shown as percentages of the initial number of animals per group. *P < 0.05, **P < 0.01 versus control NK cells.