Figure 2.
Effect of HSC on NF-κB pathway. RAW264.7, J774A.1, THP-1 cells were treated with HSC for 4 h then LPS (1 μg/mL) was added for 2 h. The protein expression of TAK1/PKC/p65 in RAW264.7 cell (A) J774A.1 cell (B) and THP-1 cell (C) by western blotting; (D, E) The localization of p65 in the cytoplasm and nucleus of RAW264.7 cells was detected by western blotting (D) and immunofluorescence (E) (Scale bar = 20 μm).; # P < 0.001 compared to control alone group; **P < 0.01, and ***P < 0.001, compared to LPS alone group, ns, no significance (n=3).