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. 2022 Feb 25;13:846384. doi: 10.3389/fimmu.2022.846384

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Copyright © 2022 Han, Yuan, Cui, He, Wang, Zhuo, Yang and Gao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of HSC on the PIP2 signaling pathway. (A) RAW264.7, J774A.1, THP-1 cells treated with HSC for 4 h, and then induced by LPS (1 μg/ml) for 1 h; (A, B, and C) The expression of PIP2, PLCγ2, DAG, and IP3 were detected by western blotting in RAW264.7 (A), J774A.1 (B), THP-1 (C) cells; (D) The PLCγ2 expression was detected by immunofluorescence assay in RAW264.7 cells; (E) Detection of PIP2 expression in RAW264.7 cells by flow cytometry; (F) Detection of PIP2 expression by immunofluorescence in RAW264.7 and THP-1 cells (Scale bar=20 μm); ^# P < 0.001 compared to control alone group; ^* P < 0.05, ^** P < 0.01, and ^*** P < 0.001, versus the LPS group, (n=3).