Figure 8.

Binding of rPoC3a to peripheral blood leukocytes (PBL). (A) PBL was incubated with or without (control) rPoC3a or rSumo, and FITC-labeled goat anti-mouse IgG was used to detect the proteins bound to the cells. The cells were stained with DAPI and observed with a confocal microscope. Bar, 10μm. (B, C) Chemotactic activity of rPoC3a. The chemotactic activity of rPoC3a in various concentrations against PBL was determined using transwell migration assay. Chemotactic index was presented as fold increase in the number of migrated cells induced by rPoC3a compared to that induced by PBS (control). Data are shown as means ± SEM (N = 3). N, the number of times the experiment was performed. **P < 0.01; *P < 0.05. (C) The migrated cells induced by the rPoC3a in (B) were observed under a microscope. Bar, 20μm.