Table 2.
Comparison of enrichment hours for detecting Salmonella Enteritidis in inoculated litter using immunomagnetic separation-multiple displacement amplification (IMS-MDA) real-time PCR.
| Salmonella Enteritidis inoculum (Av. ± S.D. CFU/g) | Ct1 values ± S.D. (detection rate %, n = 8) |
||
|---|---|---|---|
| 4 h | 6 h | 8 h | |
| 10.5 ± 1.4 | 28.7 ± 2.2 (100) | 29.01 ± 1.9 (100) | 28.9 ± 1.3 (100) |
| 1.05 ± 0.1 | 32.8 ± 0.7 (100) | 32.4 ± 1.2 (100) | 32.1 ± 1.7 (100) |
| 0.11 ± 0.01 | 32.5 ± 1.1 (50) | 33.4 ± 1.2 (50) | 32.9 ± 1.2 (100) |
1
The threshold cycle (Ct), which is the intersection between each fluorescence curve and a threshold line, was calculated using Applied Biosystems 7500 real-time PCR system software version 2.0.6 (Thermo Fisher Scientific, Waltham, MA). Negative results correspond to Ct values ≥40 or sample with Ct values higher than that of negative control.