Table 4.
Most probable number (MPN) and the number of positive samples by immunomagnetic separation-multiple displacement amplification (IMS-MDA) real-time PCR, conventional real-time PCR, and culture method in detection of Salmonella spp. in boot swab, drag swab, and litter samples from chicken farm (n = 174).
Number of positive samples |
|||
---|---|---|---|
MPN (mpn/g) | Culture method | Real-time PCR | IMS-MDA real-time PCR |
Negative | 0 | 6 | 7 |
0.03 | 2 | 1 | 1 |
0.036 | 3 | 3 | 3 |
0.062 | 2 | 2 | 2 |
0.094 | 2 | 2 | 2 |
0.29 | 1 | 1 | 1 |
0.36 | 2 | 2 | 2 |
11 | 1 | 1 | 1 |
>11 | 6 | 6 | 6 |