Table 1.
Primers used for quantitative RT-PCR.
| Primer name1 | Primer sequence2 5'-3' | Product size (bp) | GenBank accession number |
|---|---|---|---|
| β-actin | F: TGCTGTGTTCCCATCTATCG | 150 | NM_205518 |
| R: TTGGTGACAATACCGTGTTCA | |||
| IGF-1 | F: ACCTTGGCCTGTGTTTGCTTAC | 111 | NM_001004384 |
| R: AGCCTCTGTCTCCACATACGAAC | |||
| MSTN | F: TACCCGCTGACAGTGGATTTC | 153 | NM_001001461 |
| R: GCCTCTGGGATTTGCTTGG | |||
| MyoD | F:GGAGAGGATTTCCACAGACAACTC | 113 | NM_204214 |
| R: CTCCACTGTCACTCAGGTTTCCT |
1
β-actin, beta-actin; IGF-1, insulin-like growth factor-1; MSTN, myostatin; MyoD myogenic determination factor 1.
2
F, forward; R, reverse.