Table 3.
Accuracy and precision data of the method for the targeted analytes at different QC concentration levels (n = 6). For metabolites with a lower working range (neopterin, 3-hydroxykynurenine, 3-hydroxyanthranilic acid, anthranilic acid, quinolinic acid, picolinic acid and methylhistamine) only two QC concentrations were assessed.
| Analyte | Accuracy (% MRE) |
Intra-run precision(% CV) |
Inter-run precision(% CV) |
||||||
|---|---|---|---|---|---|---|---|---|---|
| 10 ng/mL | 300ng/mL | 2000 ng/mL | 10 ng/mL | 300ng/mL | 2000 ng/mL | 10 ng/mL | 300ng/mL | 2000 ng/mL | |
| Neopterin | -6.60 | -2.96 | 5.56 | 4.05 | 9.52 | 4.55 | |||
| Tryptophan | 12.3 | 6.69 | 7.97 | 11.0 | 10.6 | 7.62 | 6.59 | 8.20 | 8.53 |
| Kynurenine | -12.2 | 1.05 | 11.9 | 6.29 | 3.78 | 7.41 | 5.78 | 7.48 | 13.1 |
| Kynurenic acid | 2.29 | -12.9 | 7.09 | 4.98 | 4.02 | 3.82 | 14.8 | 12.4 | 8.42 |
| 3-hydroxykynurenine | 2.27 | 8.87 | 7.44 | 9.49 | 13.8 | 8.34 | |||
| Xanthurenic acid | -2.84 | -3.31 | 1.34 | 7.24 | 10.8 | 4.23 | 14.5 | 12.2 | 11.5 |
| Anthranilic acid | 6.76 | -4.02 | 6.08 | 8.36 | 4.08 | 2.75 | |||
| 3-hydroxyanthranilic acid | -3.96 | 10.7 | 4.90 | 6.16 | 12.3 | 8.51 | |||
| Quinolinic acid | 11.09 | 9.3 | 11.8 | 9.48 | 12.6 | 8.45 | |||
| Picolinic acid | 5.53 | 2.32 | 5.19 | 4.24 | 8.49 | 3.62 | |||
| Arginine | 15.6 | 0.10 | -6.81 | 14.9 | 8.79 | 5.24 | 8.88 | 11.3 | 12.2 |
| Citrulline | 11.8 | 12.1 | 12.3 | 5.74 | 4.26 | 8.16 | 9.89 | 8.67 | 9.95 |
| Methylhistamine | -13.5 | -10.9 | 11.6 | 12.3 | 14.1 | 6.95 | |||