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. 2022 Mar 10;139(10):1541–1556. doi: 10.1182/blood.2021012734

Figure 3.

Figure 3.

STAT3 is an essential molecule in JAK/STAT signaling in ATLL. (A) The indicated cell lines were infected with a lentivirus expressing sgSTAT3_1 and sgSTAT3_2 together with GFP. The GFP-positive cell fraction was monitored as in Figure 2A. (B) Immunoblot analysis of STAT3 protein in sgSTAT3-transduced ST1 and Jeko1. (C-D) KK1 cells were transduced with retroviruses expressing an sgRNA-resistant STAT3-WT or with empty vector (C). ST1 cells were transduced with lentiviruses expressing an sgRNA-resistant STAT3-WT, STAT3-D556N, STAT3-Y640F, STAT3-D661Y together with puromycin-resistance gene or with an empty vector expressing puromycin-resistance gene. (D) After puromycin selection of transduced cells, cells were subsequently transduced with lentiviruses coexpressing GFP and either sgSTAT3_1 or sgSTAT3_2. sgAAVS1 and sgRPL6 were used as a negative and positive control sgRNA, respectively. The GFP-positive cell fraction was monitored as in Figure 2A. (E) Immunoblot analysis of STAT3 protein in ATLL and MCL cell lines. (F) Immunoblot analysis of STAT3 protein in ST1 ATLL cells transduced with indicated cDNA-expressing vectors. Error bars represent the SEM of replicates (A,C-D).