TABLE 2.
Microbial counts expressed as log10 CFU/g of 3 replicates ± standard deviation of the main microbial groups detected in O1, O2, C5, and C8 drupe samples during the fermentation.
| Days of fermentation |
|||||
| Microbial groups | T0 | T15 | T30 | T60 | T80 |
| Enterobacteriaceae | |||||
| O1 | 4.14 ± 0.06cA | 3.41 ± 0.10bB | 2.10 ± 0.16bC | <1 | <1 |
| O2 | 3.35 ± 0.10dA | 2.61 ± 0.07cB | <1 | <1 | <1 |
| C5 | 4.83 ± 0.08aA | 4.63 ± 0.06aA | 3.56 ± 0.10aB | 2.43 ± 0.12C | <1 |
| C8 | 4.53 ± 0.06bA | 4.78 ± 0.09aB | 3.38 ± 0.09aC | 1.89 ± 0.05D | <1 |
| LAB | |||||
| O1 | 6.50 ± 0.06aC | 7.59 ± 0.09bA | 7.37 ± 0.08aB | 7.33 ± 0.05bB | 7.62 ± 0.11bA |
| O2 | 6.45 ± 0.08aC | 7.91 ± 0.08aA | 7.33 ± 0.07aB | 7.65 ± 0.05aB | 7.85 ± 0.07aA |
| C5 | 6.32 ± 0.14aA | 6.30 ± 0.08cA | 6.14 ± 0.09bA | 5.65 ± 0.05cB | 5.19 ± 0.09cC |
| C8 | 6.40 ± 0.15aA | 5.78 ± 0.09dB | 5.61 ± 0.09bB | 5.70 ± 0.06cB | 5.28 ± 0.08cB |
| Yeasts | |||||
| O1 | 2.32 ± 0.08bD | 7.52 ± 0.07bA | 7.37 ± 0.08cA | 5.70 ± 0.10cB | 4.00 ± 0.07cC |
| O2 | 2.90 ± 0.10aE | 6.85 ± 0.05dA | 6.41 ± 0.08dB | 5.23 ± 0.07dC | 3.85 ± 0.07cD |
| C5 | 2.48 ± 0.07bE | 7.10 ± 0.08cB | 7.75 ± 0.05bA | 6.79 ± 0.07aC | 5.58 ± 0.14aD |
| C8 | 2.33 ± 0.07bD | 8.08 ± 0.11aA | 7.96 ± 0.09aA | 6.08 ± 0.07bB | 5.00 ± 0.09bC |
| Mesophilic Bacteria | |||||
| O1 | 5.48 ± 0.08cA | 5.48 ± 0.10cA | 5.19 ± 0.09cB | 4.51 ± 0.08bC | 4.52 ± 0.07bC |
| O2 | 5.70 ± 0.09bA | 4.36 ± 0.07dBC | 4.23 ± 0.06dC | 4.49 ± 0.08bB | 4.35 ± 0.09bBC |
| C5 | 6.30 ± 0.09aB | 6.85 ± 0.06bA | 6.81 ± 0.08bA | 6.18 ± 0.08aB | 5.78 ± 0.08aC |
| C8 | 5.00 ± 0.08dD | 8.08 ± 0.09aA | 8.26 ± 0.07aA | 6.30 ± 0.09aB | 5.30 ± 0.09aC |
| Coagulase positive staphylococci | |||||
| O1 | 2.31 ± 0.07c | <1 | <1 | <1 | <1 |
| O2 | 2.11 ± 0.21c | <1 | <1 | <1 | <1 |
| C5 | 3.59 ± 0.09aB | 4.91 ± 0.08A | 4.95 ± 0.05A | 3.70 ± 0.10B | <1 |
| C8 | 3.23 ± 0.11bB | 4.90 ± 0.08A | 4.70 ± 0.08A | 3.33 ± 0.05B | <1 |
| Coagulase negative staphylococci | |||||
| O1 | 2.85 ± 0.06cB | 4.20 ± 0.09aA | <1 | <1 | <1 |
| O2 | 2.63 ± 0.07bA | 2.30 ± 0.09cA | <1 | <1 | <1 |
| C5 | 2.91 ± 0.06aC | 3.33 ± 0.06bB | 4.70 ± 0.08aA | 2.60 ± 0.09C | <1 |
| C8 | 2.45 ± 0.09aA | 1.54 ± 0.07dB | 2.55 ± 0.06bA | <1 | <1 |
O1, fermentation at 5% of NaCl, with the addition of L. plantarum F1.16 and F3.5 strains; O2, fermentation at 5% of NaCl, with the addition of L. plantarum C11C8, F1.16 and F3.5 strains; C5, spontaneous fermentation at 5% of NaCl; C8, spontaneous fermentation at 8% of NaCl.
a–dDifferent letters within the same column indicate significant differences at p < 0.05.
A–EDifferent letters within the same row indicate significant differences at p < 0.05.