Fig. 3.
Effects of vanilloid analogues on the TRPV1 activation by capsaicin or acids in mTRPV1-HEK. (A, B) Actual recordings of [Ca2+]i responses induced by the sequential application of capsaicin (30 nM, A) or acids (pH 5.5, B) and capsaicin (1 μM) as a control (a). VMA (1 mM) was added before, during and after the second stimuli (b). (C, D) The relative amplitude of [Ca2+]i increases induced by the second application of capsaicin or pH 5.5 (S2) to that of the first one (S1) (Cont; control, VAcid; 1 mM, VMA; 1 mM, vanillin; 1 mM, VAlc; 1 mM, and VBE; 10 μM). Columns with vertical lines show mean ± SEM (C: control; n = 100, VAcid; n = 83, VMA; n = 53, vanillin; n = 23, VAlc; n = 91, and VBE n = 9, D: control; n = 94, VAcid; n = 44, VMA; n = 27, vanillin; n = 21, VAlc; n = 40, and VBE; n = 8), *, p < 0.05, **, p < 0.01 vs. Control by one-way ANOVA with Tukey-Kramer test.