Fig. 4.

Effects of VMA on the TRPV1 activation in mouse sensory neurons. (A) Actual recordings of [Ca²⁺]_i responses induced by the sequential application of capsaicin (20 nM), capsaicin (1 μM, 1 min), and KCl (80 mM, 1 min) in mouse DRG neurons as a control (a). VMA (1 mM) was added before, during and after the second application of capsaicin (b). (B) The relative amplitude of [Ca²⁺]_i increases (S2) induced by the second application of capsaicin or pH 5.5 to that of the first one (S1). Columns with vertical lines show mean ± SEM (control of capsaicin; n = 41, VMA vs. capsaicin; n = 31, control of pH 5.5; n = 51, VMA vs. pH 5.5; n = 30), **, p < 0.01 vs Control by un-paired t-test.