Table 1.

Bacterial strains and plasmids

Strain or plasmid	Relevant characteristics*	Received year	Reference and/or source
Strain
A. baumannii
ATCC 19606(A)	Clinical isolate; type strain	2014	Beate Averhoff collection; genome accession number: CP058289.1
ATCC 19606(D)	Clinical isolate; type strain	2020	German Collection of Microorganisms and Cell Cultures, DSMZ GmbH (genome available at https://genomes.atcc.org/genomes/1577c3a70f334038)
ATCC 19606(H)	Clinical isolate; type strain	–	[25]; genome accession number: CP045110.1
ATCC 19606(M)	Clinical isolate; type strain	–	[27]; genome accession number: CP046654.1
ATCC 19606(O)	Clinical isolate; type strain	–	[26]; genome accession number: AP022836
ATCC 19606(S)	Clinical isolate; type strain	2019	Harald Seifert collection
ATCC 19606(T)	Clinical isolate; type strain	2010	Kevin Towner collection
ATCC 17978	Clinical isolate	2007	[114]
ACICU	MDR clinical isolate, prototype of the international clonal lineage II	2007	[115]
AB5075	MDR and hypervirulent clinical isolate	2019	[116]
A. baylyi BD413 (ADP1)	Naturally transformable strain	2017	[117]
Acinetobacter dijkshoorniae 271	Member of the ACB complex	2017	[118]; H. Seifert collection
Acinetobacter nosocomialis UKK_0361	Member of the ACB complex	2017	[66]; H. Seifert collection
Acinetobacter pittii UKK_0145	Member of the ACB complex	2017	[66]; H. Seifert collection
E. coli DH5α	recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 Δ(lacZYA-argF)U169 [ϕ80dlacZΔM15] F- NalR	–	[60]
Plasmid
pCR-Blunt II-TOPO	E. coli cloning vector; KmR, ZeoR	–	ThermoFisher
p1ATCC19606	Native plasmid of A. baumannii ATCC 19606T	–	[25]
pMAC	Native plasmid of A. baumannii ATCC 19606T	–	[87]
pVRL1	E. coli-Acinetobacter species shuttle vector for general cloning purposes; GmR	–	[66]
pVRL1ΔTA	pVRL1 carrying a deletion in the TA system; GmR	–	[66]
pVRL2	E. coli-Acinetobacter species shuttle vector for arabinose-inducible gene expression; GmR	–	[66]
pME6032	Broad-host-range shuttle vector for IPTG-inducible gene expression; TcR	–	[68]
pCR-p1ATCC19606	Full length p1ATCC19606 ligated to pCR-Blunt II-TOPO; KmR, ZeoR	–	This work
pCR-p1ATCC19606Δ1	Deletion derivative of p1ATCC19606 cloned into pCR-Blunt II-TOPO; KmR, ZeoR	–	This work
pCR-p1ATCC19606Δ2	Deletion derivative of p1ATCC19606 cloned into pCR-Blunt II-TOPO; KmR, ZeoR	–	This work
pCR-p1ATCC19606Δ3	Deletion derivative of p1ATCC19606 cloned into pCR-Blunt II-TOPO; KmR, ZeoR	–	This work
pCR-p1ATCC19606Δ4	Deletion derivative of p1ATCC19606 cloned into pCR-Blunt II-TOPO; KmR, ZeoR	–	This work
pCR-p1ATCC19606Δ5	Deletion derivative of p1ATCC19606 cloned into pCR-Blunt II-TOPO; KmR, ZeoR	–	This work
pCR-p1ATCC19606ΔhigB2A2	pCR-p1ATCC19606 carrying a deletion in the TA system; KmR, ZeoR	–	This work
pVRL2higA2	higA2-like antitoxin promoterless gene cloned into pVRL2; GmR	–	This work
pME6032higB2	higB2-like toxin promoterless gene cloned into pME6032; TcR	–	This work

*Nal^R, nalidixic acid resistant; Km^R, kanamicin resistant; Tc^R, tetracycline resistant; Zeo^R, zeocin resistant, Gm^R, gentamicin resistance.