Table 2.
Selected genes with high expression differences during growth on glucose (G), fructose (F), lactate (L) or a combination of substrates (LG/LF). All transcripts are listed that exhibited >5 log2-fold changes in expression, together with expression changes for linked genes within the same gene cluster. Changes of less than fivefold are listed for the Rnf and Hnd genes clusters in A soehngenii because of their metabolic relevance. All listed genes have an FDR value of <0.05. NoDiff=No significant difference in expression. Expanded to all genes in Tables S6–S11
|
log2-fold change |
||||
|
A. soehngenii L2-7 |
|
G v L |
G v LG |
L v LG |
|
L2-7_01905 |
Lactate permease (lctE) |
−14.3 |
−4.4 |
10.0 |
|
L2-7_01906 |
d-iLDH (lctD) |
−14.2 |
−3.7 |
10.5 |
|
L2-7_01907 |
ETF-B (lctB) |
−14.1 |
−3.5 |
10.6 |
|
L2-7_01908 |
ETF-A (lctC) |
−14.2 |
−3.7 |
10.5 |
|
L2-7_01909 |
Acyl-CoA dehydrogenase (lctG) |
−14.1 |
−3.7 |
10.4 |
|
L2-7_01910 |
Lactate racemase (lctF) |
−14.0 |
−3.5 |
10.5 |
|
L2-7_01911 |
LutR transcriptional regulator (lctA) |
−3.0 |
NoDiff |
2.5 |
|
L2-7_02157 |
Aquaporin family protein |
−6.3 |
−9.6 |
−3.3 |
|
L2-7_02158 |
Racemase |
−5.3 |
−9.0 |
−3.7 |
|
L2-7_02594 |
Putative cyclic lactone autoinducer peptide |
−5.2 |
NoDiff |
5.8 |
|
L2-7_02595 |
Hypothetical protein |
−4.9 |
NoDiff |
4.9 |
|
L2-7_02596 |
RNA polymerase subunit sigma-70 |
−5.7 |
NoDiff |
6.0 |
|
L2-7_02597 |
Helix-turn-helix domain-containing protein |
−5.7 |
NoDiff |
5.8 |
|
L2-7_00821 |
Hnd complex, subunit A |
−4.33 |
NoDiff |
5.12 |
|
L2-7_00822 |
Hnd complex, subunit C |
−4.07 |
NoDiff |
4.56 |
|
L2-7_00823 |
Hnd complex, subunit D |
−3.98 |
NoDiff |
4.38 |
|
L2-7_00377 |
Rnf complex, subunit C |
−2.90 |
NoDiff |
2.91 |
|
L2-7_00378 |
Rnf complex, subunit D |
−3.08 |
NoDiff |
2.98 |
|
L2-7_00379 |
Rnf complex, subunit G |
−3.20 |
NoDiff |
3.13 |
|
L2-7_00380 |
Rnf complex, subunit E |
−3.33 |
NoDiff |
3.25 |
|
L2-7_00381 |
Rnf complex, subunit A |
−3.27 |
NoDiff |
3.22 |
|
L2-7_00382 |
Rnf complex, subunit B |
−3.24 |
NoDiff |
3.15 |
|
log2-fold change |
||||
|
C. catus GD/7 |
|
F v L |
F v LF |
L v LF |
|
GD-7_01094 |
Propionyl CoA transferase (lapA) |
−10.3 |
−9.2 |
1 |
|
GD-7_01095 |
Lactoyl CoA epimerase (lapB) |
−11.8 |
−10.7 |
1.1 |
|
GD-7_01096 |
Lactoyl CoA dehydratase subunit (lapC) |
−12.6 |
−11.4 |
1.2 |
|
GD-7_01097 |
Lactoyl CoA dehydratase subunit (lapD) |
−13.1 |
−11.9 |
1.2 |
|
GD-7_01098 |
Lactoyl CoA dehydratase subunit (lapE) |
−13 |
−11.9 |
1.2 |
|
GD-7_01099 |
Lactate permease (lapF) |
−13 |
−11.9 |
1.2 |
|
GD-7_00065 |
3-hydroxybutyryl-CoA dehydratase |
−8.6 |
−6.2 |
2.5 |
|
GD-7_00066 |
H+/gluconate symporter and related permeases |
−6.9 |
−4.6 |
2.3 |
|
GD-7_00760 |
Amidohydrolase family protein |
−7.6 |
−4.4 |
3.2 |
|
GD-7_00761 |
MFS transporter |
−8.3 |
−4.7 |
3.6 |
|
GD-7_01087 |
Acyl-CoA dehydrogenase |
−9.6 |
-6 |
3.6 |
|
GD-7_01088 |
ABC transporter substrate-binding protein |
−6.6 |
−3.5 |
3.1 |
|
GD-7_01551 |
Acyl-CoA dehydrogenase |
−11.7 |
−10.8 |
1 |