Table 1.

List of libraries and summary of library preparation conditions

Library name	Barcode	Reagent	Polymerase	Ann. (°C)	Ext. (°C)	Species	BLT dilution	TWB	PCR cycles and input DNA (ng)	MiSeq cycles
											Ec.SF.B1	v0(t)	SF	EPM	62	68	E. coli	1	na	5/200	600
Ec.SF_1 : 50.B1	v0(t)	SF	EPM	62	68	E. coli	1 : 50	na	12/10	600
Ec.SF.B2	v0	SF	EPM	62	68	E. coli	1	na	5/200	600
Ec.SF_1 : 50.B2	v0	SF	EPM	62	68	E. coli	1 : 50	na	12/10	600
Ec.SF_PS.B2	v0	SF	PS 2 µl	62	68	E. coli	1	na	5/200	600
Ec.HF.B3	v0	HF	PS 1 µl	62	68	E. coli	1 : 50	Y	12/10	600
Ec.HF_55A.B2	v0	HF	PS 2 µl	55	68	E. coli	1 : 50	Y	12/10	600
Ec.HF_06x.B3	v1	HF	PS 1 µl	62	68	E. coli	1 : 50_0.6×	N	12/10	600
Ec.HF-barcode_v0(t).B0	v0(t)	HF	PS 2 µl	62	68	E. coli	1 : 50	Y	12/10	300
Ec.HF-barcode_v1.B3	v1	HF	PS 1 µl	62	68	E. coli	1 : 50	N	12/10	600
Pa.SF.B1	v0(t)	SF	EPM	62	68	P. aeruginosa	1	na	5/200	600
Pa.SF_1 : 50.B1	v0(t)	SF	EPM	62	68	P. aeruginosa	1 : 50	na	12/10	600
Pa.HF.B2	v0(t)	HF	PS 2 µl	62	68	P. aeruginosa	1 : 50	Y	12/10	600
Pa.HF_55A.B2	v0	HF	PS 2 µl	55	68	P. aeruginosa	1 : 50	Y	12/10	600
Pa.HF_55A72E.B2	v0	HF	PS 2 µl	55	72	P. aeruginosa	1 : 50	Y	12/10	600
Pa.HF_06x.B3	v1	HF	PS 1 µl	62	68	P. aeruginosa	1 : 50_0.6×	N	12/10	600
Sa.SF.B1	v0(t)	SF	EPM	62	68	S. aureus	1	na	5/200	600
Sa.SF_1 : 50.B1	v0(t)	SF	EPM	62	68	S. aureus	1 : 50	na	12/10	600
Sa.HF.B2	v0	HF	PS 2 µl	62	68	S. aureus	1 : 50	Y	12/10	600
Sa.HF_55A.B2	v0	HF	PS 2 µl	55	68	S. aureus	1 : 50	Y	12/10	600
Sa.HF_06x.B3	v1	HF	PS 1 µl	62	68	S. aureus	1 : 50_0.6×	N	12/10	600

N, No; na, Not applicable; PS, PrimeSTAR HS DNA Polymerase (Takara); Y, Yes.