Figure 4.

Adjustment of QARS1 structure by XL-MS-derived intra-links

(Top) XL-MS-derived intra-links in QARS1; cross-links between N-terminal domain (NTD) and catalytic domain (CAT) are highlighted (red). (Left) Crystal structure (PDB ID: 4YE6) of the NTD (red) and CAT (pink) of QARS1 showing 3 cross-links much greater than the ∼27 Å limit (left). (Right) Amended model of QARS1 with XL-MS-derived cross-links within distance constraints of the cross-linker. Figure reprinted with permission from Khan et al. (2020).