Cross-linking buffer (100 mL) | Final concentration | Amount |
---|---|---|
1 M HEPES (pH 7.8) | 20 mM | 2 mL |
5 M NaCl | 150 mM | 3 mL |
Add ddH2O to 100 mL. Store at 4°C for up to 3–6 months.
Cross-linking buffer (100 mL) | Final concentration | Amount |
---|---|---|
1 M HEPES (pH 7.8) | 20 mM | 2 mL |
5 M NaCl | 150 mM | 3 mL |
Add ddH2O to 100 mL. Store at 4°C for up to 3–6 months.