REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Normal rabbit IgG [Dilution (1:100)]	Cell Signaling Technology	2729S
EPRS1, rabbit polyclonal [Dilution (1:100)]	BioSynthesis	N/A
Chemicals, peptides, and recombinant proteins
1 M Tris-HCl (pH-7.5)	Boston BioProducts	BBT-75
1 M Tris-HCl (pH-7.8)	Boston BioProducts	BBT-78
5 M NaCl	Thermo Fisher Scientific	Cat#24740011
Triton X-100	Sigma-Aldrich	T8787
Bovine serum albumin	Sigma-Aldrich	A8806
Fetal Bovine Serum	Gemini	Cat#100-106
1 M HEPES (pH 7.8)	Boston BioProducts	BBH-78
1 M HEPES (pH 8.0)	Boston BioProducts	BBH-80
1 M MgCl2	Alfa Aesar	J61014
Dithiothreitol	Sigma-Aldrich	Cat#10708984001
Disuccinimidyl sulfoxide (DSSO)	Thermo Fisher Scientific	A33545
Dimethyl sulfoxide (DMSO)	Sigma-Aldrich	D8418
Ammonium bicarbonate	Sigma-Aldrich	A6141
Protease inhibitor	Thermo Fisher Scientific	78429
Acetic acid	Fisher Chemical	A113-50
Formic acid	Fisher Chemical	A117-50
Acetonitrile	Fisher Chemical	A955-4
Sequencing Grade Modified Trypsin	Promega	V5111
HBSS	Corning	T55-022-PB
Trypsin for cell culture	Caisson	T019
EDTA	Sigma-Aldrich	E6511
Sodium bicarbonate	Sigma-Aldrich	S6014
Phenol red	Caisson	P024
Penicillin	Sigma-Aldrich	P3032-100MU
Streptomycin	Gibco	11860-038
L-Glutamine	Caisson	G010
DMEM base powder	Caisson	DMP08
PBS base powder	Gibco	21600-044
12N HCl	Fisher Scientific	A144-212
Experimental models: Cell lines
Human: HEK293T	ATCC	CRL-3216
Critical commercial assays
Protein A/G magnetic beads	Thermo Fisher Scientific	Cat#88803
C18 spin columns	Thermo Fisher Scientific	Cat#89873
Mini Kleenpak	Pall	KA02DJLP2S
Supor DCF	Pall	CFS91SSPZK
Acropak 500	Pall	Cat#12995
MILLEX GP	Millipore	SLGP033RS
Software and algorithms
Proteome Discoverer	Thermo Fisher Scientific	https://planetorbitrap.com/proteome-discoverer
XlinkX	Thermo Fisher Scientific	https://www.hecklab.com/software/xlinkx/
PyMol	Schrödinger	https://pymol.org/2/
Swiss-Model	Protein Structure and Bioinformatics group	https://swissmodel.expasy.org/
PatchDock	Wolfson Lab, Tel Aviv University	https://bioinfo3d.cs.tau.ac.il/PatchDock/
Other
Microcentrifuge	Eppendorf	5424R
Minicentrifuge	Benchmark	C1008
Thermomixer	Eppendorf	Cat#4376600
DynaMag-2 magnet	Thermo Fisher Scientific	12321D
Plate reader	Molecular Devices	SpectraMax 190
Mass spectrometer	Thermo Fisher Scientific	Fusion Lumos
NanoHPLC Pump: NCS-3500RS Nano ProFlow	Thermo Fisher Scientific	5041.0010A
NanoHPLC Autosampler: WPS-3000TPL RS	Thermo Fisher Scientific	5826.0020
NanoHPLC Degasser: SRD-3400	Thermo Fisher Scientific	5035.9245
Trapping column	Thermo Fisher Scientific	Cat#164564
Analytical column	Thermo Fisher Scientific	Cat#164940