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| Trypsin-EDTA (100 mL) | Final concentration | Amount |
|---|---|---|
| HBSS | 1× | 951 mg |
| Trypsin for cell culture | 0.05% | 50 mg |
| EDTA | 0.53 mM | 17.3 mg |
| NaHCO3 | 4.16 mM | 35 mg |
| Phenol red | 0.027 mM | 1.1 mg |
Add ddH2O to 100 mL. Bring the pH to 7.0 by bubbling the solution with CO2 gas. Filter the final solution using 0.1 micron Mini Kleenpak filter. Store at 4°C for up to 2–3 weeks.
