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| L-glutamine solution (100 mL) | Final concentration | Amount |
|---|---|---|
| L-glutamine | 200 mM | 2.923g |
| NaCl | 0.85% | 0.85g |
Add ddH2O to 100 mL. The pH is ∼5.5. Filter the final solution using 0.1 micron Mini Kleenpak filter. Store at −20°C for up to 1 year. Thaw at 37°C water bath just before supplementing DMEM media.
