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| Pre-blocking buffer (10 mL) | Final concentration | Amount |
|---|---|---|
| 1 M Tris-HCl (pH 7.5) | 100 mM | 1 mL |
| 5 M NaCl | 150 mM | 0.3 mL |
| Triton X-100 | 1% | 0.11 mL |
| Bovine serum albumin (BSA) | 0.02% | 2 mg |
Add ddH2O to 10 mL. Prepare just before use as BSA is highly susceptible to microbial contamination. Add complete protease inhibitor cocktail to a 1× final concentration prior to use.
