| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Brilliant Violet 605™ anti-human CD19 antibody (dilution 1:200) | BioLegend | Cat#302243 |
| Brilliant Violet 785™ anti-human CD3 antibody (dilution 1:200) | BioLegend | Cat#317329 |
| PE/Cyanine7 anti-human IgM antibody (dilution 1:200) | BioLegend | Cat#314532 |
| Bacterial strains | ||
| One Shot™ Stbl3™ Chemically Competent E. coli | Invitrogen™ | Cat#C737303 |
| Biological samples | ||
| PBMCs | Fred Hutchinson Cancer Research Center | Specimen Processing and Research Cell Bank |
| Chemical, peptides, and recombinant proteins | ||
| DMEM, high glucose, GlutaMAX™ Supplement | Gibco | Cat#10566016 |
| IMDM, no phenol red | Gibco | Cat#21056023 |
| RPMI with Glutamine | Corning | Cat#10-040-CV |
| Hank’s Balanced Salt Solution, 1× without calcium, magnesium and phenol red (HBSS) | Corning | Cat#21-022-CV |
| EasySep™ Buffer | STEMCELL Technologies | Cat#20144 |
| Fetal bovine serum (FBS) | Sigma-Aldrich | Cat#F0926-500ML |
| Beta-mercaptoethanol | Gibco | Cat#21985-023 |
| TryplE | Thermo Fisher Scientific | Cat#12604013 |
| Penicillin-Streptomycin (10,000 U/mL) | Gibco | Cat#15140122 |
| polyL-Lysine solution | Sigma-Aldrich | Cat#P4707-50 mL |
| PBS | Cytiva | Cat#SH30256.01 |
| Cytofix/Cytoperm™ Fixation and permeabilization solution | BD Biosciences | Cat#554722 |
| Perm/Wash™ Buffer | BD Biosciences | Cat#554723 |
| Cytoperm™ Permeabilization Buffer Plus | BD Biosciences | Cat#561651 |
| AbC™ Total Antibody Compensation Bead Kit | Invitrogen | Cat#A10513 |
| Chemicals | ||
| Polybrene | Millipore Sigma | Cat#TR-1003-G |
| Sucrose | Fisher BioReagents | Cat#BP220-1 |
| Ampicillin | Sigma-Aldrich | Cat#A5354 |
| MCD40L (100 ng/mL) | Enzo Life Sciences | Cat#ALX-522-110-C010 |
| CpG ODN 2006 (1 μg/m)l | InvivoGen | Cat#NC9902842/tlrl-2006-1 |
| IL-2 (50 ng/mL) | PeproTech | Cat#200-02 |
| IL-10 (50 ng/mL) | PeproTech | Cat#200-10 |
| IL-15 (10 ng/mL) | Miltenyi Biotec | Cat#130-095-765 |
| Miller’s LB | Corning | Cat#46-050-CM |
| AF350 LIVE/DEADTM fixable violet dead cell stain (dilution 1:1000) | Thermo Fisher Scientific | Cat#L34964 |
| Critical Commercial kits | ||
| NucleoBond Xtra Midi Plus kit for transfection-grade plasmid DNA | MACHEREY-NAGEL | Cat#REF 740412.50 |
| Profection® Mammalian transfection system | Promega | Cat#E1200 |
| EasySep Human B cell Isolation Kit | STEMCELL Technologies | Cat#17954 |
| The "Big Easy" EasySep™ Magnet | STEMCELL Technologies | Cat#18001 |
| DNeasy Blood and Tissue kit | QIAGEN | Cat#69504 |
| Lenti-X p24 Rapid Titer Kit | TaKaRa Bio | Cat#632200 |
| Experimental models: Cell lines | ||
| CD46KO 293T Cells | Torbett lab ∗The Scripps Research Institute, La Jolla, CA, USA, is in the process of depositing the cell line at the National Gene Vector Biorepository (https://www.ngvbcc.org/Home.action) |
Ozog et al. (2019) |
| Nalm6 cells | Torbett lab | https://www.atcc.org/products/crl-3273 |
| Oligonucleotides | ||
| HIV-1 Fw primer | This paper | 5′-CTGTTGTGTGACTCTGGTAACT-3′ |
| HIV-1 Rv primer | This paper | 5′-TTCGCTTTCAAGTCCCTGTT-3′ |
| MKL2 Fw primer | This paper | 5′-AGATCAGAAGGGTGAGAAGAATG-3′ |
| MKL2 Rv primer | This paper | 5′-GGATGGTCTGGTAGTTGTAGTG-3′ |
| HIV-1 probe | This paper | 5′-FAM/AAATCTCTA/ZEN/GCAGTGGCGCCCG/3IABkFQ-3′ |
| MKL2 probe | This paper | 5′-HEX/TGTTCCTGC/ZEN/AACTGCAGATCCTGA/3IABkFQ-3′ |
| Recombinant DNA | ||
| Measles virus Edmonston strain hemagglutinin (pCG-HΔ24) | Ozog et al. (2019) | Genbank ID AB583749.1 |
| Measles virus Edmonston strain fusion (pCG-FΔ30) | Ozog et al. (2019) | Genbank ID U03657.1 |
| pMDLg/RRE | (Dull et al., 1998) | Addgene; #12251 |
| pRSV-Rev | (Dull et al., 1998) | Addgene; #12253 |
| p-MND-eGFP-eCD4-Ig | Torbett lab | eCD4-Ig Gardner et al., 2015 |
| Software and algorithms | ||
| Calculator for determining the number of copies of a template | Andrew Staroscik | http://cels.uri.edu/gsc/cndna.htmL |
| FlowJo 10.7.1 software | FlowJo (Becton Dickinson) | https://www.flowjo.com/ |
| GraphPad Prism 9.3.0 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| BioRender | BioRender | https://biorender.com/ |
| Quantasoft software | Bio-Rad | Cat#186-3007 |
| Other | ||
| QX100 ddPCR | Bio-Rad | Cat#186-3001 |
| QX droplet generator | Bio-Rad | Cat#186-3002 |
| QX100 droplet reader | Bio-Rad | Cat#186-3003 |
| Droplet reader oil | Bio-Rad | Cat#186-3004 |
| Droplet generation oil | Bio-Rad | Cat#186-3005 |
| PX1 PCR plate sealer | Bio-Rad | Cat#181-4000 |
| ddPCR Supermix for probes | Bio-Rad | Cat#186-3010 |
| DG8 cartridges | Bio-Rad | Cat#186-4008 |
| DG8 gaskets | Bio-Rad | Cat#186-3009 |
| Droplet generator cartridge holders | Bio-Rad | Cat#186-3051 |
| 96 well plates semi-skirted | Bio-Rad | Cat#HSL9601 |
| 0.22 μm PES filter | Millipore | Cat#SLGPR33RS |
| 14 mL polystyrene round bottom tube | Falcon | Cat#352057 |
| Ultracentrifuge tubes | Nalgene | Cat#3110-0500 |
| Bio-Rad T100 Thermal Cycler | Bio-Rad | Cat#1861096 |
| OptimaTM L-90K Ultracentrifuge | Beckman Coulter | Cat#SKU: 8043-30-1191 |
| SW28 Ti rotor | Beckman Coulter | Cat#342204 |
| BD LSRII | BD Biosciences | N/A |
| EVOS M5000 Imaging system | Thermo Fisher Scientific | Cat#AMF5000 |
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