Supplemental Figure S1.

eIF3k is not involved in the regulation of antiviral signaling.A and B, MIC cells were transfected with eIF3k or control vector. Cells were treatment with SCRV or Poly(I:C) for an additional 12 h at posttransfection. IRF3 mRNAs, and IRF3 protein were detected with qPCR and immunoblot. C, MX1 mRNAs and ISG56 mRNAs were determined by qPCR after treatment with SCRV or Poly(I:C) for an additional 12 h at post-transfection. D, EPC cells were transfected with the indicated plasmids for 24 h, SCRV or Poly(I:C) treatment was as before, followed by detection of luciferase activity. E, MIC cells were transfected with ATG5-si and Ctrl-si; and the qPCR was used to detect the knockdown efficiency. The data are shown as the mean ± SD of three independent experiments. (∗) p< 0.05, (∗∗) p< 0.01 versus the controls.