Skip to main content
. 2022 Feb 15;298(3):101730. doi: 10.1016/j.jbc.2022.101730

Figure 1.

Figure 1

eIF3k is upregulated by V. harveyi stimulation.A, M. miiuy eIF3k mRNAs were determined in different tissues by qPCR. B and C, eIF3k mRNAs in M. miiuy intestines were determined by qPCR after V. harveyi (B) or LPS (C) infection at different times. D, expression of eIF3k mRNAs in MIC was determined by qPCR after LPS stimulation at for different times. E, expression of eIF3k mRNAs was determined in intestines by qPCR after SCRV infection. F and G, expression of eIF3k mRNAs was determined in MIC by qPCR after SCRV or Poly(I:C) stimulation. H, eIF3k was activated by LPS. EPC cells were co-transfected with eIF3k reporter gene plasmid (100 ng) or control vector pGL3-Basic (100 ng). pRL-TK (10 ng) was included to normalize the expression level. Cells were treated with 10 μg/ml LPS for additional 6 h at 24 h posttransfection, followed by detection of luciferase activity. The data are shown as the mean ± SD of three independent experiments. (∗) p< 0.05, (∗∗) p < 0.01 versus the controls. eIF3k, eukaryotic translation initiation factor 3k; EPC, epithelioma papulosum cyprini; MIC, M. miiuy intestine cell.