Table 1.
Kinetic parameters of BmTrxR, OvTrxR and hTrxR1 with DTNB, hTrx1, BmTrx1, BmTrx2, BmTrxP40G, OvTrx1, and OvTrx2, calculated based on activity data from Fig. 3.
| Enzyme | Substrate | Active site | Km (μM) | kcat (min−1) | kcat/Km (min−1 μM−1) |
|---|---|---|---|---|---|
| BmTrxR | DTNB | 70 ± 7.8 | 1200 ± 57 | 17 | |
| hTrx1 | -CGPC- | 21 ± 3.7 | 430 ± 46 | 20 | |
| BmTrx1 | -CPPC- | 2.7 ± 1.4 | 380 ± 25 | 140 | |
| BmTrx2 | -CPQC- | 4.7 ± 0.6 | 680 ± 30 | 140 | |
| BmTrx P40G | -CGPC- | 4.7 ± 0.9 | 570 ± 39 | 120 | |
| OvTrx1 | -CPPC- | 1.1 ± 0.3 | 199 ± 13 | 180 | |
| OvTrx2 | -CPQC- | 9.6 ± 3.5 | 48 ± 6.9 | 5.0 | |
| OvTrxR | DTNB | 170 ± 12 | 1700 ± 34 | 10 | |
| hTrx1 | -CGPC- | 14 ± 1.4 | 720 ± 39 | 52 | |
| BmTrx1 | -CPPC- | 2.4 ± 1.2 | 440 ± 43 | 180 | |
| OvTrx1 | -CPPC- | 1.6 ± 0.4 | 301 ± 19 | 190 | |
| OvTrx2 | -CPQC- | 4.5 ± 2.2 | 50 ± 6.4 | 11 | |
| hTrxR1 | DTNB | 61 ± 8.4 | 2500 ± 120 | 42 | |
| hTrx1 | -CGPC- | 4.3 ± 1.2 | 1040 ± 79 | 242 | |
| BmTrx1 | -CPPC- | 4.4 ± 1.1 | 860 ± 59 | 200 | |
| OvTrx1 | -CPPC- | 6.1 ± 2.1 | 960 ± 110 | 157 | |
| OvTrx2 | -CPQC- | 8.5 ± 4.1 | 91 ± 16 | 11 |