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. 2022 Feb 25;9:847829. doi: 10.3389/fmolb.2022.847829

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Copyright © 2022 Wińska, Widło, Senkara, Koronkiewicz, Cieśla, Krzyśko, Skierka and Cieśla.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of CX-4945 on the level of p-p65 Ser 529 (nuclear factor kappa-light-chain-enhancer of activated B cells), DHFR, TS, SHMT1, and SHMT2 in CCRF-CEM. Western blot analysis of the following proteins in the crude extracts obtained from CCRF-CEM after 6, 16, 24, 48, and 72 h of treatment with 2, 4, and 8 µM CX-4945: (A) p-p65 Ser 529; (B) GAPDH was used as a loading control for each sample; (C) DHFR; (D) TS; (E) SHMT1; and (F) SHMT2. Preparation of cell extracts and protein detection are described in Materials and Methods. Densitometry quantifications for each tested protein were calculated with untreated cells (CTRL) serving as the reference point showing relative protein levels.