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. 2022 Feb 24;25(3):103984. doi: 10.1016/j.isci.2022.103984

Figure 2.

Figure 2

Detection of ALAHM expression in EVs of LUAD cells and EV uptake of normal liver cells

Real-time RT-PCR was used to confirm expression differences in expression levels of ALAHM and EVs-ALAHM in HPAEpiC and NCI-H1975 cells, respectively (A and B). After overexpression or knockdown of ALAHM in NCI-H1975 cells, expression levels of EVs-ALAHM were detected by real-time RT-PCR (C). EVs derived from NCI-H1975 cells were co-cultured with L02 cells. EVs uptake by L02 cells was observed under a fluorescence microscope and images were taken (D). Bar graphs represent ratios plotted as mean ± SD of three separate experiments. Compared with the control group, ∗p < 0.05 and ∗∗p < 0.01.