Antibodies |
|
Rabbit monoclonal anti-HGF |
Abcam |
RRID:ab178395 |
Rabbit monoclonal anti-CD63 |
Abcam |
RRID:ab134045 |
Rabbit monoclonal anti-TSG101 |
Abcam |
RRID:ab125011 |
Mouse monoclonal anti-Tubulin |
Abcam |
RRID:ab7291 |
Rabbit monoclonal anti-AUF1 |
Abcam |
RRID:ab259895 |
Mouse monoclonal anti-GAPDH |
Abcam |
RRID:ab8245 |
HRP-goat anti-rabbit IgG |
Thermo Scientific |
RRID:A16104 |
|
Biological samples |
|
Patients serum (gene sequencing detection) |
TUMC & Tianjin Chest Hospital |
N/A |
Patients serum (lncRNAs screening and validation) |
TUMC & Tianjin Chest Hospital |
N/A |
|
Chemicals, peptides, and recombinant proteins |
|
Primers |
GenePharma Inc. |
Syntheses |
|
Experimental models: Cell lines |
|
Human:NCI-H1975 |
ATCC |
CBP60121 |
Human:HPAEpiC |
Sciencell |
3200 |
Human:L02 |
ATCC |
CBP60224 |
|
Experimental models: Mice |
|
Balb/c nude |
Charles River Labs |
SCXK 2020-0013 |
|
Oligonucleotides |
|
siRNA (targeting ALAHM) |
GenePharma Inc. |
Syntheses |
siRNA NC |
GenePharma Inc. |
Syntheses |
|
Recombinant DNA |
|
pcDNA3.1 plasmid |
GenePharma Inc. |
Syntheses |
|
Software and algorithms |
|
ImageJ |
VILBER Co., Ltd. |
FUSION FX EDGE SPECTRA |
Graphpad Prism 6.02 |
GraphPad Software Co., Ltd. |
N/A |
TRANSFAC database |
Biostars |
https://www.biostars.org/p/69086/ |
catRAPID |
Biostars |
http://s.tartaglialab.com/page/catrapid_omics2_group |
SPSS 20.0 |
SPSS Inc. |
N/A |
|
Other |
|
RNA FISH probe |
Ruibo Biotechnology |
RRID:C10910
|
Human:ELISA kit |
Abcam |
RRID:ab267631 |
Cytoplasmic and Nuclear RNA Purification kit |
AmyJet Scientific |
RRID:NGB-21000 |
SYBR qPCR Master Mix |
Vazyme Biotech |
RRID:Q511 |
HiScript II Q Select RT SuperMix |
Vazyme Biotech |
RRID:R222-01 |
RIPA lysate |
Fount BioTech |
RRID:HC1235 |
DNA Marker |
Thermo Fisher Scientific |
RRID:SM1332 |
BCA Protein Assay kit |
Thermo Fisher Scientific |
RRID:23227 |
Dimethylsulfoxide |
Sigma-Aldrich |
RRID:D8418 |
Fetal bovine serum |
Gibco |
RRID:12484010 |
Trypsin-EDTA |
Gibco |
RRID:25200072 |
DMEM |
Gibco |
RRID:72400047 |
Database: Sequence data |
This paper |
NCBI:SUB10791255 |