Fig. 1. Global DNA 5hmC deposition and TET family expression in CD4⁺ T cells of AR patients. (A) After positive selection using anti-human CD4 magnetic nanoparticles, the positive and negative fractions were verified by flow cytometry. (B) Global DNA 5hmC deposition in CD4⁺ T cells was detected by dot blot and quantified by average gray value of dot. (C) Expression of CD4 and TET2 in human nasal tissues was indicated by immunofluorescence. The immunofluorescence intensity of TET2 was calculated in manually selected CD4⁺cells. (D) Relative mRNA expression of the TET family (TET1, TET2, and TET3) in CD4⁺ T cells was measured by RT-qPCR and calculated using comparative Ct values after normalizing to β-actin. Bars indicate mean ± standard error of the mean.

Control, healthy volunteers; AR, allergic rhinitis; 5hmC, 5-hydroxymethylcytosine; TET, ten-eleven translocation; RT-qPCR, reverse real-time quantitative transcription polymerase chain reaction.

^*P < 0.05, ^**P < 0.01.