Fig. 3. Validating the expressions of DhMG in CD4⁺ T cells of AR patients and that of healthy volunteers. (A) The normalized read count for 5hmC around DhMG including CD300H, IGF1R, ITCH, PRKDC, SCN1B, and TSC22D1 is shown. The DhMR are highlighted in red. (B) Relative mRNA expressions of CD300H, IGF1R, ITCH, PRKDC, SCN1B, and TSC22D1 in CD4⁺ T cells were measured by RT-qPCR and calculated using comparative Ct values after normalizing to β-actin. Bars show mean ± standard error of the mean.

DhMG, differentially hydroxymethylated genes; Control, healthy volunteers; AR, allergic rhinitis; 5hmC, 5-hydroxymethylcytosine; IGF1R, insulin-like growth factor 1 receptor; ITCH, itchy E3 ubiquitin protein ligase; PRKDC, protein kinase, DNA-activated, catalytic subunit; SCN1B, sodium voltage-gated channel beta subunit 1; TSC22D1, transforming growth factor β-stimulated clone 22 domain family member 1; RT-qPCR, reverse real-time quantitative transcription polymerase chain reaction.

^*P < 0.05, ^**P < 0.01.