Fig. 4. Tet2 deficiency exacerbates allergic inflammation in OVA-exposed mice. (A) OVA-exposed mice models of AR were developed using WT and KO mice via intraperitoneal sensitization followed by intranasal administration of OVA. (B) Symptoms of nose scratching and sneezing were observed in 5 minutes after the final challenge. (C) Histopathology of nasal mucosae in mice was evaluated with hematoxylin and eosin staining. Eosinophils in nasal mucosae were counted in high-power fields at 400× magnification. (D) OVA-sIgE and Th-related cytokines in serum or NLF are shown.

Tet, ten-eleven translocation; OVA, ovalbumin; AR, allergic rhinitis; WT, wild type; KO, Tet2 knockout; PBS, phosphate-buffered saline; NLF, nasal lavage fluid; OVA-sIgE, ovalbumin-specific immunoglobulin E; NS, not significant.

^*P < 0.05, ^**P < 0.01.