Fig. 5. Tet2 deficiency aggravates the imbalance between Th1/Th2 and Treg/Th17 cells in OVA-exposed mice. (A) Lymphocytes were extracted from fresh the mouse spleens and the expressions of anti-IFN-γ, IL-4, FOXP3 and IL-17A in gated CD4⁺ T cells were analyzed. (B) The percentages of CD4⁺IFN-γ⁺Th1 cells, CD4⁺IL-4⁺Th2 cells, CD4⁺FOXP3⁺Treg cells, and CD4⁺IL-17⁺Th17 cells in CD4⁺ cells were calculated. (C) The ratio of Treg/Th17 cells to that of Th1/Th2 cells is shown.

Tet, ten-eleven translocation; Th, T helper; Treg, regulatory T; PBS, phosphate-buffered saline; OVA, ovalbumin; WT, wild type; KO, Tet2 knockout; NS, not significant.

^*P < 0.05, ^**P < 0.01.