TABLE 2.
PCR and sequence primers used in this study
| Primera | Sequence | Positionsb |
|---|---|---|
| Tn1546 primers | ||
| 22.F | 5′-GGATTTACAACGCTAAGCC | 22–40 |
| 184.R | 5′-ACCATATGTCGCCCTTAG | 184–167 |
| 934.F | 5′-TGTGGATTTGCATCTGC | 934–950 |
| 1009.R | 5′-ACGGTACAACATCTTCGTC | 1009–991 |
| 1292.R | 5′-TTACTCATGGATGTGGCC | 1292–1275 |
| 1723.F | 5′-ACAGGTGAGTCATCAGGC | 1723–1740 |
| 1890.F | 5′-TAAATAATCATAGTCGGCAGG | 1890–1910 |
| 1913.R | 5′-CGTCCTGCCGACTATG | 1913–1898 |
| 1924.R | 5′-TAGGAACTTGCACGTCCT | 1924–1908 |
| 2768.F | 5′-AGGATGGACTAACACCAATC | 2768–2787 |
| 2880.R | 5′-TGCTGTTCAATTAGCTGTTC | 2880–2861 |
| 3514.F | 5′-ACTGTAATGGCTGGTGTTAAC | 3514–3534 |
| 3560.R | 5′-TATCCGAATAAGATCTCGCT | 3560–3542 |
| 3940.R | 5′-ATTTATCAGATTATAGGGCCG | 3940–3920 |
| 3992.F | 5′-TTATTGTGGATGATGAACATG | 3992–4012 |
| 4426.F | 5′-AACGAGAAGCAGTTATCCC | 4426–4444 |
| 4511.R | 5′-TCGGAGCTAACCACATTC | 4511–4494 |
| 5235.F | 5′-ATATCACGTTGGACAAAGC | 5235–5253 |
| 5374.R | 5′-TTCATCGGTCATCTGCAC | 5374–5357 |
| 5747.F | 5′-ACGTTTAGGGTAGAGCTTCC | 5747–5766 |
| 6039.F | 5′-GTTTATGGATGTGAGCAGG | 6039–6057 |
| 6113.R | 5′-TATCGTTGCCATAACGC | 6113–6097 |
| 6964.F | 5′-AAAGGAGACAGGAGCATG | 6964–6981 |
| 7035.R | 5′-TTACGTCATGCTCCTCTGAG | 7035–7017 |
| 7486.R | 5′-CAAAAACAGGATAGGTAAACG | 7486–7466 |
| 7875.F | 5′-CCGCATTGTACTGAACG | 7875–7891 |
| 7986.R | 5′-CAAGCGGTCAATCAGTTC | 7986–7969 |
| 8544.F | 5′-GCATATAGCCTCGAATGG | 8544–8561 |
| 8691.R | 5′-TTACATACGTCGGGTTTCC | 8691–8673 |
| 8969.R | 5′-GATTGTGCCGTTTTGC | 8969–8954 |
| 9519.F | 5′-ACCAGCAGGTTATAGTGAGC | 9519–9538 |
| 9580.R | 5′-TCGTCAAGCTTGATCCTAC | 9580–9562 |
| 9970.R | 5′-GCCATCCTTACCTCCTTG | 9970–9953 |
| 10716.R | 5′-TTTTCCCCTCACTTCACAC | 10716–10698 |
| 10778.F | 5′-TTTAGTGCTGAGGAATTGG | 10778–19796 |
| IS1216V primers | ||
| IS1216V.A | 5′-GGAAAGCAATTTCAGCAG | 254–271 |
| IS1216V.B | 5′-TCGATGCAGATGGTTTAAC | 516–534 |
| IS1216V.C | 5′-CACTTGTAATAGAGGGGGC | 659–641 |
| IS1216V.D | 5′-TGGGATTCCCAATAATACC | 895–913 |
| IS1216V.E | 5′-AGCTTAAATCATAGATACCGTAAGG | 913–935 |
| IS1216V.F | 5′-TTCATCGTCATTCCTCCTCCTG | 243–225 |
a
The names of the Tn1546 primers indicate the position of the first nucleotide and the orientation of the primer (F, forward; R, reverse).