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Comparison of larger signals obtained using the manual method (Sm) and the automated method (Sa) for quantifying the pixel intensity. Correlation plots when the spots are shaped like a large circle (A), or a large rectangle (B), circles are the mean and error bars are the standard deviation (n = 3). Standard deviation (σ) associated with colorimetric signals S obtained with the proposed software (black) or with the manual method (red) (C,D).
