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Analysis of recombinant HLA-A2 α chain and β2m expression by Coomassie Blue-Stained SDS-PAGE
A distinct band of 35.9 kDa and 14 kDa can be observed for insoluble fractions (35.9 kDa and 14 kDa bands are circled with a red rectangle in the picture). BI, before IPTG induce. AI, after IPTG induce. SN, supernatant after ultrasound centrifugation. P, pellet after ultrasound centrifugation. M, molecular weight marker.
